Molecular Detection for Genotype In Sheep Infection with Echinococcus Granulosus at Holy Kerbala Province
DOI:
https://doi.org/10.59675/V123Keywords:
PCR , Hydatid cysts, Echinococcus Granulosus, KerbalaAbstract
Hydatidosis due to cystic Echinococcosis is one of the most important public health and economic problems in different countries including Iraq”. The study was conducted the prevalence of Hydatid cysts disease in sheep in slaughter houses in the holy city of Kerbala "and to detect the rates of infection with E. granulosus genotype using optimal (PCR) technique with specific designing primer for cystic fluid" . The study was carried out between October 2021 and April 2022 . A total number of 93 sheep were examined via autopsy for hydatid cysts in slaughterhouses in Kerbala , including both sexes and different ages , then we used molecular genotype sequencing technique , specific PCR primer design . "Our PCR results of the study showed that cystic echinococcosis genotypes was 24 (53.33%) were detected as Echinococcus granulosus isolates which isolated from internal organs and the percentage were significantly increases as 49 (52.68%), 33 (35.48 %), 11 (11.82 %) for liver, lung and liver with lung respectively" .
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