Evaluate the Concordance Between Two Diagnostic Methods, RT-PCR And ELISA Techniques for the Detection of CCHFV in Karbala City
DOI:
https://doi.org/10.59675/V217UKeywords:
CCHFV, Ticks and ELISA, RT-PCR.Abstract
Crimean-Congo haemorrhagic fever virus (CCHFV) is a widespread pathogen virus transmitted by ticks that causes a severe disease known as Crimean-Congo haemorrhagic fever in animals and humans. This study aims to investigate the occurrence of CCHFV in ticks and bovines in different regions of Karbala City/Iraq. Tick and blood samples were collected from four cattle farms, and molecular RT-PCR and serological analyses were conducted. The serum sodium concentration was calculated to compare infected and non-infected cattle with CCHFV. The study identified five tick genera, with Rhipicephalus (Boophilus) microplus and Amblyomma variegatum being the most prevalent species. However, no ticks tested positive for CCHFV. Serological analysis of the blood samples showed that none was positive for IgM antibody, but 19.00% tested positive for IgG antibodies and 29.3% for both IgG and IgM. The seroprevalence was higher in cattle from Ayn al-time (36.70%) and Hindiya (16.70 %) compared to other regions, the study found fair concordance between the two serological and molecular assays used for diagnosis of CCHFV virus; as well as the study concluded that sodium levels are an important prognostic factor for CCHFV infection, cattle with CCHFV.
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