Isolation And Identification of Campylobacter Coli _Campylobacter Lari from Humans, Local Milk, and Milk Products Using Classical And Molecular Techniques In Karbala Province
DOI:
https://doi.org/10.59675/V117Keywords:
Campylobacter coli & campylobacter lari, infection rate, Milk& milk product, Antimicrobial susceptibility, Kerbala, retail pointsAbstract
This study, which ran from October 2022 to the end of March 2023, examined the frequency of Campylobacter species in samples of human, milk, and milk products in the province of Karbala. as well as the detection of the antibiotic sensitivity of these isolates. A total of one hundred (100) samples were collected systematically, including 100 samples from children at the General Children's Hospital in Kerbala (Iraq) who were experiencing diarrhoea, fever, and abdominal pain, as well as 50 samples of raw milk and 50 samples of milk products from various local shops and farms distributed throughout the province of Karbala. In order to identify the pathogen, samples were promptly inoculated into enriched and subcultured on selective media. On Campylobacter agar base selective medium, Campylobacter spp. Colonies showed up as tiny; mucoid colonies frequently generate separate colonies that are flat with uneven borders and non-hemolytic at 24 to 48 hours. These colonies are typically greyish in appearance, while some must be creamy grey. The polymerase chain reaction method identified ten isolates out of 150 samples (6.6%) as Campylobacter species.
The ten isolates were subjected to sequencing to detect Campylobacter species, which found two species (C. coli _ C. lari). Results showed that Campylobacter spp. Out of eight positive isolates, campylobacter coli recorded 8 (80%), while campylobacter lari recorded (20%) positive results. Campylobacter ssp displayed 100% sensitivity to azithromycin, tetracycline, ampicillin, gentamycin, and imipenem in an antibiotic susceptibility test using the disk diffusion assay. Due to Campylobacter's vulnerability to the aforementioned antibiotics, frequently employed as the preferred treatment for campylobacteriosis, the resistance pattern of this bacteria shown in this study is highly concerning. These levels of incidence and resistance call for more investigation and effective defences because they could pose health dangers to the public
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