Histopathological Assessments on Kidney and Spleen of Experimentally Infected Male Rats by Staphylococcus aureus: Antibiotics versus Probiotics
DOI:
https://doi.org/10.59675/V312Keywords:
Pathogenic bacteria, Histological features, Linezolid, Probiotics, Enterotoxin.Abstract
Background: Staphylococcus aureus (S. aureus) stands as a prominent contributor to infections and fatalities on a global scale. Antibiotic resistance added a layer of complexity to the management of S. aureus infections. Presently, there is no viable vaccination option. Probiotics have been identified as possible agents that can help overcome antibiotic resistance. Aim: In the current study aimed to investigate the histological changes in organs such as the kidney and spleen in male rats after infection with 10 μL S. aureus (1.0 × 107 CFU/mL) intraperitoneally and treated with a daily dose of 1 ml of (floratil) yeast suspension, daily oral dose of (1 ml) of probiotic colloid for 7 days, and compared with the group inoculated intraperitoneally injections of 1 mL of an antibiotic suspension (linezolid tablets) for 7 days. Results: the animals treated with probiotics showed more significant histopathological alterations and recovery as compared to antibiotics-treated groups in response to restoring renal and splenic tissue from the histological effects caused by bacterial infection; this study shows the protective effects of antibiotics, probiotics, and yeasts in maintaining kidney and spleen tissue structures after S. aureus infection histologically and to compare between them. Conclusion: this study investigated the protective effects of probiotics on renal and splenic tissues against Staphylococcus aureus to the extent that they are more effective than antibiotics in histological damage recovery.
References
Cheung GYC, Bae JS, and Otto M. Pathogenicity and virulence of Staphylococcus aureus. Virulence, 2021;12(1):547-569, DOI: 10.1080/21505594.2021.1878688
Lindsay JA, and Holden MT. Staphylococcus aureus: Superbug, super genome? rends Microbiol. 2004;12: 378–385.
Schito GC. The importance of the development of antibiotic resistance in Staphylococcus aureus. Clin. Microbiol. Infect. 2006;12: S3-S8.
Cuny C, and Wieler LH, Livestock-Associated WW. MRSA: the Impact on Humans. Antibiotics (Basel). 2015;4(4):521-543.
Mrochen DM, de Oliveira FLM, Raafat D, Holtfreter S. Staphylococcus aureus host tropism and its implications for murine infection models. International journal of molecular sciences. 2020; 2119:7061.
Abbas LA, Rady AM. Isolation and molecular detection of Staphylococcus aureus from feline otitis in Baghdad. Iraqi Journal of Veterinary Sciences. 2023; 37(Supplement I-IV):141-146. doi: 10.33899/ijvs.2023.1375560.2696
Weese JS. Methicillin-resistant Staphylococcus aureus in animals. ILAR J. 2010; 51: 233-244.
Waldvogel FA, Mandell GL, Douglas RG, Bennett JE. Staphylococcus aureus. Principles and practices of infectious disease. 3rd Edn. 1990:1754-1777.
DeLeo FR, Otto M, Kreiswirth BN. Community-associated meticillin-resistant Staphylococcus aureus. Lancet. 2010;375(9725):1557–1568.
Howden BP, Giulieri SG, Fok W, and Lung T. Staphylococcus aureus host interactions and adaptation. Nat Rev Microbiol. 2023; 21:380–395. https://doi.org/10.1038/s41579-023-00852-y
Lister JL, Horswill AR. Staphylococcus aureus biofilms: recent developments in biofilm dispersal. Front. Cell Infect. Microbiol. 2014; 4:178.
Foster TJ. The MSCRAMM Family of Cell-Wall-Anchored Surface Proteins of Gram-Positive Cocci. Trends Microbiol. 2019;27(11):927-941.
Cho JS, Pietras EM, Garcia NC. IL17 is essential for host defense against cutaneous Staphylococcus aureus infection in mice. J Clin Invest. 2010;120(5):1762-1773.
Vestergaard M, Frees D, Ingmer H. Antibiotic resistance and the MRSA problem. Microbiol Spectr. 2019;7(2):1-13. DOI: 10.1128/microbiolspec.GPP3-0057-2018
Lakhundi S, Zhang K. Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology. Clin. Microbiol. Rev. 2018; 31: e00020-18.
Kim S, Moon A. Drug induced nephrotoxicity and its biomarkers. Biomol Ther. 2012;20(3):268-272. DOI: 10.10.4062molther.20.3.268
Abdullah AT, Ahmed MA. Ameliorative role of Arabic gum against nephrotoxicity induced by ciprofloxacin in rats. Iraqi Journal of Veterinary Sciences. 2021; 35(4): 789-798. doi: 10.33899/ijvs.2020.127441.1503
Dantes R, Mu Y, Belflower R, Aragon D, Dumyati G, Harrison LH, Lessa FC, ynfield R, Nadle J, Petit S, Ray SM, Schaffner W, Townes J, Fridkin S. Emerging infections program–active bacterial core surveillance MRSA, surveillance investigators national burden of invasive methicillin-resistant Staphylococcus aureus infections, United States, 2011. JAMA Intern Med. 2013;173: 1970-1978
Piewngam P, Zheng Y, Nguyen TH. Pathogen elimination by probiotic Bacillus via signaling interference. Nature. 2018;562(7728):532-537.
Dickey SW, Cheung GYC, Otto M. Different drugs for bad bugs: antivirulence strategies in the age of antibiotic resistance. Nat Rev Drug Discov. 2017;16(7):457-471.
Rabetafika HN, Razafindralambo A, Ebenso B, Razafindralambo HL. Probiotics as Antibiotic Alternatives for Human and Animal Applications. Encyclopedia. 2023;3: 561-581. https://doi.org/10.3390/ encyclopedia3020040.
Dawood MAO, Koshio S. Recent advances in the role of probiotics and prebiotics in carp aquaculture: A review. Aquaculture. 2016; 454:243-251.
Palanivelu J, Thanigaivel S, Vickram S, Dey N, Mihaylova D, Desseva I. Probiotics in functional foods: Survival assessment and approaches for improved viability. Appl Sci. 2022;12(1):455. DOI: 10.3390/app12010455
Hassanen EI, Ragab E. In Vivo and In Vitro Assessments of the Antibacterial Potential of Chitosan-Silver Nanocomposite Against Methicillin-Resistant Staphylococcus aureus–Induced Infection in Rats. Biol Trace Elem Res. 2021; 199: 244-257 .https://doi.org/10.1007/s12011-020-02143-6
Al-Nuaimi AJ, Jabber EJ, Jawad MJ, Hassan MS, Jihad AJ, Al-Shuwaili AK. Effects of methotrexate on hepatic and testicular tissues in male rabbits: Histological, hormonal and biochemical analysis. Iraqi Journal of Veterinary Sciences. 2023; 37(Supplement I-IV): 211-217. doi: 10.33899/ijvs.2023.138717.2828
Kolaczkowska E, Kubes P. Neutrophil recruitment and function in health and inflammation. Nat Rev Immunol. 2013; 13:159-175.
Thammavongsa V, Missiakas DM, Schneewind O. Staphylococcus aureus degrades neutrophil extracellular traps to promote immune cell death. Science. 2013 15; 342 (6160):863-6. doi: 10.1126/science.1242255. PMID: 24233725; PMCID: PMC4026193.
Cheng AG, Kim HK, Burts ML, Krausz T, Schneewind O, Missiakas DM. Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. FASEB J. 2009;23(10):3393-404. doi: 10.1096/fj.09-135467.
Sheehan AA, Khudor MH, Isihak FA. Some immunological responses in rats injected with prepared bacterin toxoid of local methicillin resistant Staphylococcus aureus. Iraqi Journal of Veterinary Sciences, 2022; 36(2): 401-406. doi: 10.33899/ijvs.2021.130401.1815
Zghair ZR, Salema LH, Hana AK. Study Of Histopathological Effect of Staphylococcus aureus In Female Albino Mice. journal of Kerbala university. 2013; 9(1):85-94.
Anita R, Neelima KR, Kaur Jaspreet. Therapeutic effect of propolis on Staphylococcus aureus induced oxidative stress in spleen of Balb/c mice: A biochemical and histopathological study. Indian Journal of Natural Products and Resources (IJNPR).2022; 13(3): 346-355. DOI: 10.56042/ijnpr.v13i3.51887.
Mohmmed E, Efkairin S, Abdraba I, Eldurssi I, Bofarda E. Histopathological changes in mice spleen induced by the toxicity of Staphylococcus aureus. 2023; 19:104-115.
Al-Mathkhury HJ, Abdul-Ghaffar SN. Urinary tract infections caused by Staphylococcus aureus DNA in comparison to the candida albicans DNA. N Am J Med Sci. 2011 Dec;3(12):565-9. doi: 10.4297/najms.2011.3562.
Peng J, Lu Q, Yuan L, Zhang H. Synthetic Cationic Lipopeptide Can Effectively Treat Mouse Mastitis Caused by Staphylococcus aureus. Biomedicines. 2023;11:1188. https://doi.org/10.3390/biomedicines11041188 .
Chifiriuc MC, Stecoza C, Dracea O, Larion C, Israil A. Antimicrobial activity of some new O-acyloximino-dibenzo [b, e] thiepins and O-acyloximino-dibenzo [b, e] thiepin-5, 5-dioxides against planktonic cells. Biotechnol. Lett., 2010; 15(2):5134-5139.
Paik W. Protection from Staphylococcus Aureus Bloodstream Infection by Probiotic Exopolysaccharide. Dissertations. 2019;3361. https://ecommons.luc.edu/luc_diss/3361
Darbandi A, Asadi A, Ari MM, Ohadi E, Talebi M, Zadeh MH, Emamie AD, Ghanavati R, Kakanj M. Bacteriocins: Properties and potential use as antimicrobials. J Clin Lab Anal. 2022; 36(1): e24093.doi: 10.1002/jcla.24093
Ruiz M, Owatari M, Yamashita M, Ferrarezi J, Garcia P, Cardoso L, Martins M, Mouriño J. Histological effects on the kidney, spleen, and liver of Nile tilapia Oreochromis niloticus fed different concentrations of probiotic Lactobacillus plantarum. Tropical Animal Health and Production. 2020;52. 10.1007/s11250-019-02001-1.
Bendjeddou K, Hamma-Faradji S, Meddour AA, Belguesmia Y, Cudennec B, Bendali F, Daube G, Taminiau B, Drider D. Gut microbiota, body weight and histopathological examinations in experimental infection by methicillin-resistant Staphylococcus aureus: antibiotic versus bacteriocin. Beneficial Microbes. 2021; 15;12(3):295-305.
Saettone V, Biasato I, Radice E, Schiavone A, Bergero D, Meineri G. State-of-the-Art of the Nutritional Alternatives to the Use of Antibiotics in Humans and Monogastric Animals. Animals. 2020; 10: 2199. https://doi.org/10.3390/ani10122199.
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